La Crosse ( lə-KROSS) is a city and the county seat of La Crosse County, Wisconsin, United States. Positioned alongside the Mississippi River, La Crosse is the largest city on Wisconsin's western border. La Crosse's population as of the 2020 census was 52,680. The city forms the core of and is the principal city in the La Crosse–Onalaska Metropolitan Area, which includes all of La Crosse County and Houston County, Minnesota, with a population of 139,627. La Crosse's economy serves as a regional educational, medical, manufacturing, and transportation hub for Western Wisconsin producing a gross domestic product (GDP) of $9.7 billion as of 2022. The La Crosse area is home to the headquarters or regional offices of Kwik Trip, Organic Valley, Mayo Clinic, Gundersen Health System, Gensler, Logistics Health Incorporated, La Crosse Technology, City Brewing Company, and Trane. La Crosse County is a top ten tourist destination in the state with $433 million in travel related spending generated in 2023. La Crosse is a college town with nearly 20,000 students and home to the nationally ranked University of Wisconsin-La Crosse, Viterbo University, and Western Technical College.